Sequencing:Article Title: Hypoxia inducible factors regulate hepatitis B virus replication by activating the basal core promoter
Article Snippet: 2016 Guidotti, LG JVirol 1995 HBV1.3.32 M/F 12-15 weeks 1.4 Sequence based reagents Name Sequence Supplier siRNA HIF-1α ND Ambion; 4390824; s6539 siRNA HIF2α (EPAS1) ND Ambion; 4390824; s4700 siRNA Scrambled (Negative Control) ND Ambion; 4390843 siRNA HIF1b (ARNT1) ND Santa Cruz; sc-29733 Silencer Negative Control No.1 siRNA ND Ambion; AM4611 Mouse HIF-1b 1 ND ThermoFisher; AM16708 Mouse HIF-1b 2 ND ThermoFisher; AM16708 Negative control siRNA-1 ND ThermoFisher; AM4404021 Updated version November 2018 HBV-pgRNA TaqMan® Gene Expression Assay ND ThermoFisher; AIKAMSS CAIX TaqMan® Gene Expression Assay ND ThermoFisher; Hs00154208_m1 VEGFA TaqMan® Gene Expression Assay ND ThermoFisher; Hs00900055_m1 B2M Control Mix ND Applied Biosystems; 4325797 PrP forward TGCTGGGAAGTGCCATGAG PrP reverse CGGTGCATGTTTTCACGATAGTA rcDNA forward GTTGCCCGTTTGTCCTCTAATTC rcDNA reverse GGAGGGATACATAGAGGTTCCTTGA cccDNA forward GCCTATTGATTGGAAAGTATGT cccDNA reverse AGCTGAGGCGGTATCTA 4T PCR T1 forward GGGGAACTAATGACTCTAGCTACC 4T PCR T1 reverse TTTAGGCCCATATTAGTGTTGACA 4T PCR T2 forward CAAGGTAGGAGCTGGAGCATTC 4T PCR T2 reverse GAGGCAGGAGGCGGATTTG 4T PCR T3 forward CTCCAGTTCAGGAACAGTAAACCC 4T PCR T3 reverse AGGAATCCTGATGTGATGTTCTCC 4T PCR T4 forward ACGGGGCGCACCTCTCTTTA 4T PCR T4 reverse GTGAAGCGAAGTGCACACGG HepBT1g forward AGACCACCAAATGCCCCTATC HepBT1g reverse TTGAGATCTTCTGCGACGCG HBV4F TTTCACCTCTGCCTAATCATCTCT HBV4R CTTTATAAGGGTCGATG CCATGC CAIX forward TATCTGCACTCCTGCCCTCTG CAIX reverse CACAGGGTGTCAGAGAGGGTGT VEGF forward TTGCCTTGCTGCTCTACCTCCA VEGF reverse GATGGCAGTAGCTGCGCTGATA NDRG1 forward TTTGATGTCCAGGAGCAGGA NDRG1 reverse ATGCCGATGTCATGGTAGGT B2M forward CTACACTGAATTCACCCCCACTG B2M reverse ACCTCCATGATGCTGCTTACATG B-Actin forward CCAACCGCGAGAAGATGA B-Actin reverse CCAGAGGCGTACAGGGATAG Mouse HIF-1b forward CTCACGAAGGTCGTTCATCTGC Mouse HIF-1b reverse CCACAAAGTGAGGTTCTCCTTCC Mouse VEGFA forward CTGCTGTAACGATGAAGCCCTG Mouse VEGFA reverse GCTGTAGGAAGCTCATCTCTCC Mouse PHD2 forward GGCGAACGATTGAGGCTTCCTT Mouse PHD2 reverse GCTGGTGACAGCAAAGAGAAGG Mouse B-Actin forward CATTGCTGACAGGATGCAGAAGG Mouse B-Actin reverse TGCTGGAAGGTGGACAGTGAGG ChIP HepB1 forward GTCTGTGCCTTCTCATCTGCC Updated version November 2018 ChIP HepB1 reverse AGACCTTGGGCAATATTTGGTGG ChIP HepB3 forward GGGGAGGAGATTAGGTTAAAGGTC ChIP HepB3 reverse CAAGAGATGATTAGGCAGAGGTGA ChIP cccDNA forward CCGTGTGCACTTCGCTTCA Werle-Lapostolle B, Gastro.
Negative Control:Article Title: Hypoxia inducible factors regulate hepatitis B virus replication by activating the basal core promoter
Article Snippet: 2016 Guidotti, LG JVirol 1995 HBV1.3.32 M/F 12-15 weeks 1.4 Sequence based reagents Name Sequence Supplier siRNA HIF-1α ND Ambion; 4390824; s6539 siRNA HIF2α (EPAS1) ND Ambion; 4390824; s4700 siRNA Scrambled (Negative Control) ND Ambion; 4390843 siRNA HIF1b (ARNT1) ND Santa Cruz; sc-29733 Silencer Negative Control No.1 siRNA ND Ambion; AM4611 Mouse HIF-1b 1 ND ThermoFisher; AM16708 Mouse HIF-1b 2 ND ThermoFisher; AM16708 Negative control siRNA-1 ND ThermoFisher; AM4404021 Updated version November 2018 HBV-pgRNA TaqMan® Gene Expression Assay ND ThermoFisher; AIKAMSS CAIX TaqMan® Gene Expression Assay ND ThermoFisher; Hs00154208_m1 VEGFA TaqMan® Gene Expression Assay ND ThermoFisher; Hs00900055_m1 B2M Control Mix ND Applied Biosystems; 4325797 PrP forward TGCTGGGAAGTGCCATGAG PrP reverse CGGTGCATGTTTTCACGATAGTA rcDNA forward GTTGCCCGTTTGTCCTCTAATTC rcDNA reverse GGAGGGATACATAGAGGTTCCTTGA cccDNA forward GCCTATTGATTGGAAAGTATGT cccDNA reverse AGCTGAGGCGGTATCTA 4T PCR T1 forward GGGGAACTAATGACTCTAGCTACC 4T PCR T1 reverse TTTAGGCCCATATTAGTGTTGACA 4T PCR T2 forward CAAGGTAGGAGCTGGAGCATTC 4T PCR T2 reverse GAGGCAGGAGGCGGATTTG 4T PCR T3 forward CTCCAGTTCAGGAACAGTAAACCC 4T PCR T3 reverse AGGAATCCTGATGTGATGTTCTCC 4T PCR T4 forward ACGGGGCGCACCTCTCTTTA 4T PCR T4 reverse GTGAAGCGAAGTGCACACGG HepBT1g forward AGACCACCAAATGCCCCTATC HepBT1g reverse TTGAGATCTTCTGCGACGCG HBV4F TTTCACCTCTGCCTAATCATCTCT HBV4R CTTTATAAGGGTCGATG CCATGC CAIX forward TATCTGCACTCCTGCCCTCTG CAIX reverse CACAGGGTGTCAGAGAGGGTGT VEGF forward TTGCCTTGCTGCTCTACCTCCA VEGF reverse GATGGCAGTAGCTGCGCTGATA NDRG1 forward TTTGATGTCCAGGAGCAGGA NDRG1 reverse ATGCCGATGTCATGGTAGGT B2M forward CTACACTGAATTCACCCCCACTG B2M reverse ACCTCCATGATGCTGCTTACATG B-Actin forward CCAACCGCGAGAAGATGA B-Actin reverse CCAGAGGCGTACAGGGATAG Mouse HIF-1b forward CTCACGAAGGTCGTTCATCTGC Mouse HIF-1b reverse CCACAAAGTGAGGTTCTCCTTCC Mouse VEGFA forward CTGCTGTAACGATGAAGCCCTG Mouse VEGFA reverse GCTGTAGGAAGCTCATCTCTCC Mouse PHD2 forward GGCGAACGATTGAGGCTTCCTT Mouse PHD2 reverse GCTGGTGACAGCAAAGAGAAGG Mouse B-Actin forward CATTGCTGACAGGATGCAGAAGG Mouse B-Actin reverse TGCTGGAAGGTGGACAGTGAGG ChIP HepB1 forward GTCTGTGCCTTCTCATCTGCC Updated version November 2018 ChIP HepB1 reverse AGACCTTGGGCAATATTTGGTGG ChIP HepB3 forward GGGGAGGAGATTAGGTTAAAGGTC ChIP HepB3 reverse CAAGAGATGATTAGGCAGAGGTGA ChIP cccDNA forward CCGTGTGCACTTCGCTTCA Werle-Lapostolle B, Gastro.
Expressing:Article Title: Hypoxia inducible factors regulate hepatitis B virus replication by activating the basal core promoter
Article Snippet: 2016 Guidotti, LG JVirol 1995 HBV1.3.32 M/F 12-15 weeks 1.4 Sequence based reagents Name Sequence Supplier siRNA HIF-1α ND Ambion; 4390824; s6539 siRNA HIF2α (EPAS1) ND Ambion; 4390824; s4700 siRNA Scrambled (Negative Control) ND Ambion; 4390843 siRNA HIF1b (ARNT1) ND Santa Cruz; sc-29733 Silencer Negative Control No.1 siRNA ND Ambion; AM4611 Mouse HIF-1b 1 ND ThermoFisher; AM16708 Mouse HIF-1b 2 ND ThermoFisher; AM16708 Negative control siRNA-1 ND ThermoFisher; AM4404021 Updated version November 2018 HBV-pgRNA TaqMan® Gene Expression Assay ND ThermoFisher; AIKAMSS CAIX TaqMan® Gene Expression Assay ND ThermoFisher; Hs00154208_m1 VEGFA TaqMan® Gene Expression Assay ND ThermoFisher; Hs00900055_m1 B2M Control Mix ND Applied Biosystems; 4325797 PrP forward TGCTGGGAAGTGCCATGAG PrP reverse CGGTGCATGTTTTCACGATAGTA rcDNA forward GTTGCCCGTTTGTCCTCTAATTC rcDNA reverse GGAGGGATACATAGAGGTTCCTTGA cccDNA forward GCCTATTGATTGGAAAGTATGT cccDNA reverse AGCTGAGGCGGTATCTA 4T PCR T1 forward GGGGAACTAATGACTCTAGCTACC 4T PCR T1 reverse TTTAGGCCCATATTAGTGTTGACA 4T PCR T2 forward CAAGGTAGGAGCTGGAGCATTC 4T PCR T2 reverse GAGGCAGGAGGCGGATTTG 4T PCR T3 forward CTCCAGTTCAGGAACAGTAAACCC 4T PCR T3 reverse AGGAATCCTGATGTGATGTTCTCC 4T PCR T4 forward ACGGGGCGCACCTCTCTTTA 4T PCR T4 reverse GTGAAGCGAAGTGCACACGG HepBT1g forward AGACCACCAAATGCCCCTATC HepBT1g reverse TTGAGATCTTCTGCGACGCG HBV4F TTTCACCTCTGCCTAATCATCTCT HBV4R CTTTATAAGGGTCGATG CCATGC CAIX forward TATCTGCACTCCTGCCCTCTG CAIX reverse CACAGGGTGTCAGAGAGGGTGT VEGF forward TTGCCTTGCTGCTCTACCTCCA VEGF reverse GATGGCAGTAGCTGCGCTGATA NDRG1 forward TTTGATGTCCAGGAGCAGGA NDRG1 reverse ATGCCGATGTCATGGTAGGT B2M forward CTACACTGAATTCACCCCCACTG B2M reverse ACCTCCATGATGCTGCTTACATG B-Actin forward CCAACCGCGAGAAGATGA B-Actin reverse CCAGAGGCGTACAGGGATAG Mouse HIF-1b forward CTCACGAAGGTCGTTCATCTGC Mouse HIF-1b reverse CCACAAAGTGAGGTTCTCCTTCC Mouse VEGFA forward CTGCTGTAACGATGAAGCCCTG Mouse VEGFA reverse GCTGTAGGAAGCTCATCTCTCC Mouse PHD2 forward GGCGAACGATTGAGGCTTCCTT Mouse PHD2 reverse GCTGGTGACAGCAAAGAGAAGG Mouse B-Actin forward CATTGCTGACAGGATGCAGAAGG Mouse B-Actin reverse TGCTGGAAGGTGGACAGTGAGG ChIP HepB1 forward GTCTGTGCCTTCTCATCTGCC Updated version November 2018 ChIP HepB1 reverse AGACCTTGGGCAATATTTGGTGG ChIP HepB3 forward GGGGAGGAGATTAGGTTAAAGGTC ChIP HepB3 reverse CAAGAGATGATTAGGCAGAGGTGA ChIP cccDNA forward CCGTGTGCACTTCGCTTCA Werle-Lapostolle B, Gastro.
Polymerase Chain Reaction:Article Title: Hypoxia inducible factors regulate hepatitis B virus replication by activating the basal core promoter
Article Snippet: 2016 Guidotti, LG JVirol 1995 HBV1.3.32 M/F 12-15 weeks 1.4 Sequence based reagents Name Sequence Supplier siRNA HIF-1α ND Ambion; 4390824; s6539 siRNA HIF2α (EPAS1) ND Ambion; 4390824; s4700 siRNA Scrambled (Negative Control) ND Ambion; 4390843 siRNA HIF1b (ARNT1) ND Santa Cruz; sc-29733 Silencer Negative Control No.1 siRNA ND Ambion; AM4611 Mouse HIF-1b 1 ND ThermoFisher; AM16708 Mouse HIF-1b 2 ND ThermoFisher; AM16708 Negative control siRNA-1 ND ThermoFisher; AM4404021 Updated version November 2018 HBV-pgRNA TaqMan® Gene Expression Assay ND ThermoFisher; AIKAMSS CAIX TaqMan® Gene Expression Assay ND ThermoFisher; Hs00154208_m1 VEGFA TaqMan® Gene Expression Assay ND ThermoFisher; Hs00900055_m1 B2M Control Mix ND Applied Biosystems; 4325797 PrP forward TGCTGGGAAGTGCCATGAG PrP reverse CGGTGCATGTTTTCACGATAGTA rcDNA forward GTTGCCCGTTTGTCCTCTAATTC rcDNA reverse GGAGGGATACATAGAGGTTCCTTGA cccDNA forward GCCTATTGATTGGAAAGTATGT cccDNA reverse AGCTGAGGCGGTATCTA 4T PCR T1 forward GGGGAACTAATGACTCTAGCTACC 4T PCR T1 reverse TTTAGGCCCATATTAGTGTTGACA 4T PCR T2 forward CAAGGTAGGAGCTGGAGCATTC 4T PCR T2 reverse GAGGCAGGAGGCGGATTTG 4T PCR T3 forward CTCCAGTTCAGGAACAGTAAACCC 4T PCR T3 reverse AGGAATCCTGATGTGATGTTCTCC 4T PCR T4 forward ACGGGGCGCACCTCTCTTTA 4T PCR T4 reverse GTGAAGCGAAGTGCACACGG HepBT1g forward AGACCACCAAATGCCCCTATC HepBT1g reverse TTGAGATCTTCTGCGACGCG HBV4F TTTCACCTCTGCCTAATCATCTCT HBV4R CTTTATAAGGGTCGATG CCATGC CAIX forward TATCTGCACTCCTGCCCTCTG CAIX reverse CACAGGGTGTCAGAGAGGGTGT VEGF forward TTGCCTTGCTGCTCTACCTCCA VEGF reverse GATGGCAGTAGCTGCGCTGATA NDRG1 forward TTTGATGTCCAGGAGCAGGA NDRG1 reverse ATGCCGATGTCATGGTAGGT B2M forward CTACACTGAATTCACCCCCACTG B2M reverse ACCTCCATGATGCTGCTTACATG B-Actin forward CCAACCGCGAGAAGATGA B-Actin reverse CCAGAGGCGTACAGGGATAG Mouse HIF-1b forward CTCACGAAGGTCGTTCATCTGC Mouse HIF-1b reverse CCACAAAGTGAGGTTCTCCTTCC Mouse VEGFA forward CTGCTGTAACGATGAAGCCCTG Mouse VEGFA reverse GCTGTAGGAAGCTCATCTCTCC Mouse PHD2 forward GGCGAACGATTGAGGCTTCCTT Mouse PHD2 reverse GCTGGTGACAGCAAAGAGAAGG Mouse B-Actin forward CATTGCTGACAGGATGCAGAAGG Mouse B-Actin reverse TGCTGGAAGGTGGACAGTGAGG ChIP HepB1 forward GTCTGTGCCTTCTCATCTGCC Updated version November 2018 ChIP HepB1 reverse AGACCTTGGGCAATATTTGGTGG ChIP HepB3 forward GGGGAGGAGATTAGGTTAAAGGTC ChIP HepB3 reverse CAAGAGATGATTAGGCAGAGGTGA ChIP cccDNA forward CCGTGTGCACTTCGCTTCA Werle-Lapostolle B, Gastro.
Chromatin Immunoprecipitation:Article Title: Hypoxia inducible factors regulate hepatitis B virus replication by activating the basal core promoter
Article Snippet: 2016 Guidotti, LG JVirol 1995 HBV1.3.32 M/F 12-15 weeks 1.4 Sequence based reagents Name Sequence Supplier siRNA HIF-1α ND Ambion; 4390824; s6539 siRNA HIF2α (EPAS1) ND Ambion; 4390824; s4700 siRNA Scrambled (Negative Control) ND Ambion; 4390843 siRNA HIF1b (ARNT1) ND Santa Cruz; sc-29733 Silencer Negative Control No.1 siRNA ND Ambion; AM4611 Mouse HIF-1b 1 ND ThermoFisher; AM16708 Mouse HIF-1b 2 ND ThermoFisher; AM16708 Negative control siRNA-1 ND ThermoFisher; AM4404021 Updated version November 2018 HBV-pgRNA TaqMan® Gene Expression Assay ND ThermoFisher; AIKAMSS CAIX TaqMan® Gene Expression Assay ND ThermoFisher; Hs00154208_m1 VEGFA TaqMan® Gene Expression Assay ND ThermoFisher; Hs00900055_m1 B2M Control Mix ND Applied Biosystems; 4325797 PrP forward TGCTGGGAAGTGCCATGAG PrP reverse CGGTGCATGTTTTCACGATAGTA rcDNA forward GTTGCCCGTTTGTCCTCTAATTC rcDNA reverse GGAGGGATACATAGAGGTTCCTTGA cccDNA forward GCCTATTGATTGGAAAGTATGT cccDNA reverse AGCTGAGGCGGTATCTA 4T PCR T1 forward GGGGAACTAATGACTCTAGCTACC 4T PCR T1 reverse TTTAGGCCCATATTAGTGTTGACA 4T PCR T2 forward CAAGGTAGGAGCTGGAGCATTC 4T PCR T2 reverse GAGGCAGGAGGCGGATTTG 4T PCR T3 forward CTCCAGTTCAGGAACAGTAAACCC 4T PCR T3 reverse AGGAATCCTGATGTGATGTTCTCC 4T PCR T4 forward ACGGGGCGCACCTCTCTTTA 4T PCR T4 reverse GTGAAGCGAAGTGCACACGG HepBT1g forward AGACCACCAAATGCCCCTATC HepBT1g reverse TTGAGATCTTCTGCGACGCG HBV4F TTTCACCTCTGCCTAATCATCTCT HBV4R CTTTATAAGGGTCGATG CCATGC CAIX forward TATCTGCACTCCTGCCCTCTG CAIX reverse CACAGGGTGTCAGAGAGGGTGT VEGF forward TTGCCTTGCTGCTCTACCTCCA VEGF reverse GATGGCAGTAGCTGCGCTGATA NDRG1 forward TTTGATGTCCAGGAGCAGGA NDRG1 reverse ATGCCGATGTCATGGTAGGT B2M forward CTACACTGAATTCACCCCCACTG B2M reverse ACCTCCATGATGCTGCTTACATG B-Actin forward CCAACCGCGAGAAGATGA B-Actin reverse CCAGAGGCGTACAGGGATAG Mouse HIF-1b forward CTCACGAAGGTCGTTCATCTGC Mouse HIF-1b reverse CCACAAAGTGAGGTTCTCCTTCC Mouse VEGFA forward CTGCTGTAACGATGAAGCCCTG Mouse VEGFA reverse GCTGTAGGAAGCTCATCTCTCC Mouse PHD2 forward GGCGAACGATTGAGGCTTCCTT Mouse PHD2 reverse GCTGGTGACAGCAAAGAGAAGG Mouse B-Actin forward CATTGCTGACAGGATGCAGAAGG Mouse B-Actin reverse TGCTGGAAGGTGGACAGTGAGG ChIP HepB1 forward GTCTGTGCCTTCTCATCTGCC Updated version November 2018 ChIP HepB1 reverse AGACCTTGGGCAATATTTGGTGG ChIP HepB3 forward GGGGAGGAGATTAGGTTAAAGGTC ChIP HepB3 reverse CAAGAGATGATTAGGCAGAGGTGA ChIP cccDNA forward CCGTGTGCACTTCGCTTCA Werle-Lapostolle B, Gastro.
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